New paper - Simulated early Earth geochemistry fuels a hydrogen-dependent primordial metabolism

Vanessa Helmbrecht, Robert Reichelt, Dina Grohmann & William D. Orsi

Nature Ecology & Evolution https://doi.org/10.1038/s41559-025-02676-w

Abstract

Molecular hydrogen is the electron donor for the ancient exergonic reductive acetyl-coenzyme A pathway (acetyl-CoA pathway), which is used by hydrogenotrophic methanogenic archaea. How the presence of iron-sulfides influenced the acetyl-CoA pathway under primordial early Earth geochemistry is still poorly understood. Here we show that the iron-sulfides mackinawite (FeS) and greigite (Fe3S4), which formed in chemical garden experiments simulating geochemical conditions of the early Archaean eon (4.0–3.6 billion years ago), produce abiotic H2 in sufficient quantities to support hydrogenotrophic growth of the hyperthermophilic methanogen Methanocaldococcus jannaschii. Abiotic H2 from iron-sulfide formation promoted CO2 fixation and methanogenesis and induced overexpression of genes encoding the acetyl-CoA pathway. We demonstrate that H2 from iron-sulfide precipitation under simulated early Earth hydrothermal geochemistry fuels a H2-dependent primordial metabolism.

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